Ultrathin coatings from isocyanate terminated star PEG prepolymers: patterning of proteins on the layers

Search Swinburne Research Bank

Home

List of Titles

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/216061

Check Swinburne's subscriptions for full text availability

Title: Ultrathin coatings from isocyanate terminated star PEG prepolymers: patterning of proteins on the layers
Author(s): Groll, Juergen; Haubensak, Wulf; Ameringer, Thomas; Moeller, Martin
Abstract: This study presents the easy and fast patterning of low molecular weight molecules that act as binding partners for proteins on Star PEG coatings. These coatings are prepared from isocyanate terminated star shaped prepolymers and form a highly cross-linked network on the substrate in which the stars are connected via urea groups and free amino groups are present. Streptavidin has been patterned on these layers by microcontact printing (μCP) of an amino reactive biotin derivative and consecutive binding of streptavidin to the biotin. Patterns of Ni 2+-nitriltriacetic acid (NTA) receptors have been prepared by printing amino functional NTA molecules in freshly prepared Star PEG layers that still contain amino reactive isocyanate groups. Complexation of the NTA groups with Ni(II) ions enabled the binding of His-tag enhanced green fluorescent protein (EGFP) in the desired pattern on the substrates. Since the unmodified Star PEG layers prevent unspecific protein adsorption, His-EGFP could selectively be bound to the sample by immersion into crude, nonpurified His-tag EGFP containing cell lysate.
Publication type: Journal article
Source: Langmuir, Vol. 21, no. 7 (Feb 2005), pp. 3076-3083
Publication year: 2005
Keyword(s): Amino groups; Binding energy; Biotin; Biotinamide; Carboxylic acids; Crosslinking; Drug derivatives; Enhanced green fluorescent protein; Isocyanates; Isocyanic acid derivative; Macrogol derivatives; Molecular structure; Molecular weight; Nonfouling surfaces; Plastic coatings; Polyethylene glycols; Proteins; Prepolymers; Ultrathin coatings
Publisher: American Chemical Society
ISSN: 0743-7463
Publisher URL: http://dx.doi.org/10.1021/la047438n
Peer reviewed