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Home List of Titles Acoustic wave immunosensing of a meningococcal antigen using gold nanoparticle-enhanced mass sensitivity
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/218460
- Acoustic wave immunosensing of a meningococcal antigen using gold nanoparticle-enhanced mass sensitivity
- Reddy, Sreenivasulu B.; Mainwaring, David E.; Al Kobaisi, Mohammad; Zeephongsekul, Panlop; Fecondo, John V.
- Bacterial meningitis is an infection of the thin membranes covering the brain and spinal cord by a number of microorganisms including Neisseria meningitidis, which can lead to permanent neurological damage in the event of late diagnosis. Given the quick onset and severity of the disease, there is a clear need for a rapid, sensitive and specific diagnostic technique. Here, we describe the development and evaluation of an acoustic wave sensor, the quartz crystal microbalance (QCM), as a rapid immunosensor employing antibodies against the cell surface outer membrane protein 85 (OMP85) of N. meningitidis as an immobilized selective layer. These antibodies were directionally orientated as receptors by thin film deposition of structured polyvinylidene fluoride and Protein A. The sensitivity of this QCM immunosensor was further increased by conjugation of the OMP85 antigen to 50 nm gold nanoparticles providing reproducible detection of the target down to 300 ng/mL. Subsequent treatment of the QCM surface with an acidic glycine solution regenerated the immunosensor allowing each crystal to be used several times.
- Publication type
- Journal article
- Biosensors and Bioelectronics, Vol. 31, no. 1 (Jan 2012), pp. 382-387
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry; 0903 Biomedical Engineering; 1007 Nanotechnology
- AuNP; Gold nanoparticle; Immunosensors; Meningococcal; Neisseria meningitidis; Polyvinylidene difluoride; PVDF; QCM; Quartz crystal microbalance
- Publisher URL
- Copyright © 2011 Elsevier B.V. All rights reserved.
- Peer reviewed