Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/219804

Title

Identification of PIP aquaporin genes from wheat

Author(s)

Yousif, Sh. A.;  Bhave, M.

Abstract

There is strong evidence that water channel proteins 'aquaporins (AQPs)' are central components in plant-water relations as well as a number of other physiological parameters. We had previously reported the isolation of 24 plasma membrane intrinsic protein (PIP) type AQPs. However, the gene numbers in rice and the polyploid nature of bread wheat indicated a high probability of further genes in the latter. The present work focused on identification of further AQP isoforms in bread wheat. With the use of altered primer design, we identified five genes homologous, designated PIP 1 ;5b, PIP2;9b, TaPIP2;2, TaPIP2;2a, TaPIP2;2b. Sequence alignments indicate PIP 1;5b, PIP2;9b are likely to be homeologues of two previously reported genes while the other three are new genes and could be homeologs of each other. The results indicate further AQP diversity in wheat and the sequence data will enable physical mapping of these genes to identify their genomes as well as genetic to determine their association with any quantitative trait loci (QTLs) associated with plant-water relation such as salinity or drought tolerance.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre

Source

World Academy of Science, Engineering and Technology, no. 59 (Nov 2011), pp. 2192-2196

Publication year

2011

FOR Code(s)

060702 Plant Cell and Molecular Biology;  060705 Plant Physiology

Keyword(s)

Aquaporins;  Homeologues;  PIP;  Plasma membrane intrinsic proteins;  Wheat

Publisher

World Academy of Science, Engineering and Technology

ISSN

2010-376X

Publisher URL

http://www.waset.org/journals/waset/v59.php

Copyright

Copyright © 2011.

Peer reviewed