Highly selective trapping of enteropathogenic E. coli on Fabry-Perot sensor mirrors

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Title: Highly selective trapping of enteropathogenic E. coli on Fabry-Perot sensor mirrors
Author(s): Ivanova, Elena P.; Truong, Vi Khanh; Gervinskas, Gediminas; Mitik-Dineva, Natasa; Day, Daniel; Jones, Robert T.; Crawford, Russell J.; Juodkazis, Saulius
Abstract: Untreated recycled water, such as sewage and graywater, will almost always contain a wide range of agents that are likely to present risks to human health, including chemicals and pathogenic microorganisms. The microbial hazards, such as large numbers of enteric pathogens that can cause gastroenteric illness if ingested, are the main cause of concern for human health. The presence of the enteropathogenic Escherichia coli (EPEC) serotype is of particular concern, as this group of bacteria is responsible for causing severe infant and travelers’ diarrhea, gastroenteritis and hemolytic uremic syndrome. A biosensing system based on an optical Fabry–Pérot (FP) cavity, capable of directly detecting the presence of EPEC within 5 min, has been developed using a simple micro-thin double-sided adhesive tape and two semi-transparent FP mirror plates. The system utilizes a poly(methyl methacrylate) (PMMA) or glass substrates sputtered by 40-nm-thick gold thin films serving as FP mirrors. Mirrors have been activated using 0.1 M mercaptopropionic acid, influencing an immobilization density of the translocated intimin receptor (TIR) of 100 ng/cm2. The specificity of recognition was confirmed by exposing TIR functionalized surfaces to four taxonomically related and/or distantly related bacterial strains. It was found that the TIR-functionalized surfaces did not show any bacterial capture for these other bacterial strains within a 15 min incubation period.
Publication type: Journal article
Research centre: Swinburne University of Technology. Faculty of Life and Social Sciences
Research centre: Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics
Source: Biosensors and Bioelectronics, Vol. 35, no. 1 (May 2012), pp. 369-375
Publication year: 2012
FOR Code(s): 0301 Analytical Chemistry; 0903 Biomedical Engineering; 1007 Nanotechnology
Keyword(s): Enteropathogenic E. coli; EPEC; Escherichia coli; Fabry-Perot sensors; Rapid detection; Translocated intimin receptor
Publisher: Elsevier
ISSN: 0956-5663
Publisher URL: http://dx.doi.org/10.1016/j.bios.2012.03.019
Additional information: The authors acknowledge support from the Australian Research Council (ARC).
Peer reviewed