Validation and quantitation of an in vitro M-cell model

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Title: Validation and quantitation of an in vitro M-cell model
Author(s): Tyrer, Peter; Foxwell, A. Ruth; Kyd, Jennelle; Harvey, Matthew; Sizer, Phillip; Cripps, Allan
Abstract: This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. alpha5beta1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro.
Publication type: Journal article
Source: Biochemical and Biophysical Research Communications, Vol. 299, no. 3 (Dec 2002), pp. 377-383
Publication year: 2002
FOR Code(s): 0304 Medicinal and Biomolecular Chemistry; 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics
Keyword(s): Caco-2; Follicle-associated epithelium; M-cell; Peyer's patch; Vaccine
Publisher: Elsevier
ISSN: 0006-291X
Publisher URL: http://dx.doi.org/10.1016/s0006-291x(02)02631-1
Peer reviewed