Home List of Titles A demonstration of the use of ultra-performance liquid chromatography-mass spectrometry [UPLC/MS] in the determination of amphetamine-type substances and ketamine for forensic and toxicological analysis
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/227468
- A demonstration of the use of ultra-performance liquid chromatography-mass spectrometry [UPLC/MS] in the determination of amphetamine-type substances and ketamine for forensic and toxicological analysis
- Apollonio, Luigino G.; Pianca, Dennis J.; Whittall, Ian R.; Maher, William A.; Kyd, Jennelle M.
- We have recently seen the emergence of ultra-performance liquid chromatography (UPLC) coupled to mass spectrometry as an alternative to traditional high-performance liquid chromatography techniques. The strengths of UPLC technology promote the ability to separate and identify drug compounds with significant gains in resolution and sensitivity and marked reductions in the overall time of analysis. As increased throughput is the desire of the practical toxicology laboratory, the aim of this study was to trial commercially available technology by assessment of the separation of several commonly encountered amphetamine-type substances. From injection of a poly-drug reference standard and whole blood extract, we successfully separated and identified amphetamine, methamphetamine, ephedrine, pseudoephedrine, phentermine, MDA, MDMA, MDEA and ketamine in less than 3 min using the Acquity UPLC-Micromass Quattro Micro API MS instrumentation (Waters Corporation, USA). In addition to this significant reduction in overall run time, all peaks exhibited acceptable resolution using selected ion recording (SIR), with analysis indicating the capability to separate 5-11 peaks in 1.75 min using the current system parameters. From this introductory data, it is therefore indicated that the technological advancements defining ultra-performance liquid chromatography will allow it to serve as a powerful analytical tool for rapid throughput analysis.
- Publication type
- Journal article
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 836, no. 1-2 (May 2006), pp. 111-115
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry; 0601 Biochemistry and Cell Biology; 1115 Pharmacology and Pharmaceutical Sciences
- Amines; Amphetamines; Amphetamine-type substances; Blood sampling; Diagnostic accuracy; Drug products; Ephedrine; Forensic identification; Forensic medicine; High performance liquid chromatography; High throughput screening; Ketamine; Liquid chromatography; Mass spectrometry; MDMA; Methamphetamine; Phase separation; Phentermine; Pseudoephedrine; Ultraviolet spectrophotometry; Toxicity testing; Ultra-performance liquid chromatography; UPLC
- Publisher URL
- Copyright © 2006 Elsevier B.V. All rights reserved.
- Peer reviewed