Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/227503

Title

Investigation of mucosal immunisation in pulmonary clearance of Moraxella (Branhamella) catarrhalis

Author(s)

Kyd, Jennelle;  John, Anna;  Cripps, Allan;  Murphy, Timothy F.

Abstract

Moraxella (Branhamella) catarrhalis is a common cause of otitis media in children and respiratory infection in adults with chronic obstructive pulmonary disease. To identify immune responses that may facilitate the development of a mucosal vaccine, a mouse model to study pulmonary responses was established. Regimes involving intra-Peyer's patch, intratracheal and intranasal routes of immunisation with killed M. catarrhalis were investigated. A mucosal immunisation regime of a primary intra-Peyer's patch immunisation with an intratracheal boost resulted in significantly enhanced pulmonary clearance of bacteria compared to controls following an intratracheal challenge with live bacteria. Additional intratracheal boosts did not induce further enhancement of clearance. Intra-Peyer's patch immunisation alone, intratracheal and intranasal immunisations did not induce enhanced clearance. The levels of specific IgG and IgA in serum and bronchoalveolar lavage fluid correlated with pulmonary clearance. The present study showed that mucosal immunisation induced enhanced pulmonary clearance of M. catarrhalis following live bacterial challenge. This mucosal immunisation model has demonstrated that a mucosal vaccine, particularly an oral vaccine, would be feasible.

Publication type

Journal article

Source

Vaccine, Vol. 18, no. 5-6 (Oct 1999), pp. 398-406

Publication year

1999

FOR Code(s)

06 Biological Sciences;  07 Agricultural and Veterinary Sciences;  11 Medical and Health Sciences

Keyword(s)

Antibodies;  Antibody response;  Attenuated vaccines;  Enzyme linked immunosorbent assay;  Immunisation;  immunoglobulins;  Inactivated vaccine;  Intestine mucosa;  Lung infection;  Lung lavage;  Moraxella (Branhamella) catarrhalis;  Moraxella catarrhalis;  Mucosal immunity;  Peyer's patches;  Phagocytes;  Respiratory tract infections;  Vaccines

Publisher

Elsevier

ISSN

0264-410X

Publisher URL

http://dx.doi.org/10.1016/s0264-410x(99)00262-5

Copyright

Copyright © 1999 Elsevier Science Ltd.

Research Projects

Immunisation strategies to prevent respiratory tract infections, National Health and Medical Research Council grant number 981963

Peer reviewed