Immune response mechanisms against Pseudomonas aeruginosa associated with mucosal immunization with protein antigens in a rat model of acute lung infection

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Title: Immune response mechanisms against Pseudomonas aeruginosa associated with mucosal immunization with protein antigens in a rat model of acute lung infection
Author(s): Thomas, Linda D.; Cripps, Allan W.; Kyd, Jennelle M.
Abstract: Pseudomonas aeruginosa is a major cause of nosocomal and community acquired chronic infections in subjects with compromised respiratory function. The microbe is environmentally ubiquitious and has a high level of innate antimicrobial resistance. This has led researchers to investigate vaccine and immunotherapeutic approaches to prevent and treat P. aeruginosa infections. Seven cytosolic non-integral proteins were studied as vaccine candidates in an acute lung infection model in the rat. Five of these (amidase, amidopeptidase, KatE, KatE and Pa13 a novel 13 kDa protein) enhanced bacterial clearance from the lung compared to control animals following challenge and are worthy of further study. Immune mechanisms stimulated by these proteins in response to both immunization and infection varied. The most pronounced degree of bacterial clearance from the lung was associated with antigens, which demonstrated greater surface exposure and induced an increase in phagocyte recruitment, in particular, an increased proportion of polymorphonuclear leukocytes. Lymphocytic proliferation and specific antibody responses in the absence of enhanced clearance were less informative as immune correlates.
Publication type: Journal article
Source: Vaccine: incorporating papers from the 6th World Congress on Vaccines, Immunisation and Immunotherapy, Milan, Italy, 23-25 September 2008, Vol. 27, no. 25-26 (May 2009), pp. 3324-3330
Publication year: 2009
FOR Code(s): 06 Biological Sciences; 07 Agricultural and Veterinary Sciences; 11 Medical and Health Sciences
Keyword(s): Immune response; Immunisation; Lung infection; Protein antigens; Pseudomonas aeruginosa
Publisher: Elsevier
ISSN: 0264-410X
Publisher URL: http://dx.doi.org/10.1016/j.vaccine.2009.01.085
Additional information: This research was supported by a grant from the National Health and Medical Research Council.
Peer reviewed