Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/227507

Title

Comparison of mucosal and parenteral immunisation in two animal models of pneumococcal infection: otitis media and acute pneumonia

Author(s)

Cripps, Allan W.;  Kyd, Jennelle M.

Abstract

Bacterial clearance and immune responses in a mouse model of pneumonia and a rat model of otitis media following parenteral or mucosal immunisation in both models were compared. Both the immunisation routes were equally effective in inducing bacterial clearance from the lung, upregulated the recruitment of white cells and lead to an increase in the concentration of TNF-α, IL-1β and specific antibody in the bronchoalveolar lavage. Both the routes of immunisation enhanced clearance of bacteria from the middle ear. Parenteral immunisation was most effective overall in enhancing bacterial clearance and recruiting white cells to the middle ear. Both the routes significantly suppressed the levels of TNF-α in the middle ear lavage. Mucosal immunisation induced a Streptococcus pneumoniae-specific IgA antibody response. Both the animal models gave highly reproducible disease and provided high levels of sensitivity for testing the efficacy of candidate vaccine antigens. Differences observed in the inflammatory responses require further study.

Publication type

Journal article

Source

Vaccine: incorporating the proceedings of the 5th International Symposium on Pneumococci and Pneumococcal Diseases, Alice Springs, Northern Territory, Australia, 02-06 April 2006, Vol. 25, no. 13 (Mar 2007), pp. 2471-2477

Publication year

2007

FOR Code(s)

06 Biological Sciences;  07 Agricultural and Veterinary Sciences;  11 Medical and Health Sciences

Keyword(s)

Animal models;  Immunisation;  Otitis media;  Pneumonia;  Streptococcus pneumoniae;  S. pneumoniae

Publisher

Elsevier

ISSN

0264-410X

Publisher URL

http://dx.doi.org/10.1016/j.vaccine.2006.09.022

Copyright

Copyright © 2006 Elsevier Ltd. All rights reserved.

Peer reviewed