Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating

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Title: Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating
Author(s): Subbiahdoss, Guruprakash; Pidhatika, Bidhari; Coullerez, Geraldine; Charnley, Mirren; Kuijer, Roel; van der Mei, Henry C.; Textor, Marcus; Busscher, Henk J.
Abstract: Biomaterials-associated-infections (BAI) are serious complications in modern medicine. Although non-adhesive coatings, like polymer-brush coatings, have been shown to prevent bacterial adhesion, they do not support cell growth. Bi-functional coatings are supposed to prevent biofilm formation while supporting tissue integration. Here, bacterial and cellular responses to poly(ethylene glycol) (PEG) brush-coatings on titanium oxide presenting the integrin-active peptide RGD (arginine-glycine-aspartic acid) (bioactive 'PEG-RGD') were compared to mono-functional PEG brush-coatings (biopassive 'PEG') and bare titanium oxide (TiO2) surfaces under flow. Staphylococcus epidermidis ATCC 35983 was deposited on the surfaces under a shear rate of 11 s-1 for 2 h followed by seeding of U2OS osteoblasts. Subsequently, both S. epidermidis and U2OS cells were grown simultaneously on the surfaces for 48 h under low shear (0.14 s-1). After 2 h, staphylococcal adhesion was reduced to 3.6-/+1.8 x 103 and 6.0-/+3.9 x 103 cm-2 on PEG and PEG-RGD coatings respectively, compared to 1.3-/+0.4 x 105 cm-2 for the TiO2 surface. When allowed to grow for 48 h, biofilms formed on all surfaces. However, biofilms detached from the PEG and PEG-RGD coatings when exposed to an elevated shear (5.6 s-1) U2OS cells neither adhered nor spread on PEG brush-coatings, regardless of the presence of biofilm. In contrast, in the presence of biofilm, U2OS cells adhered and spread on PEG-RGD coatings with a significantly higher surface coverage than on bare TiO2. The detachment of biofilm and the high cell surface coverage revealed the potential significance of PEG-RGD coatings in the context of the 'race for the surface' between bacteria and mammalian cells.
Publication type: Journal article
Source: European Cells and Materials, Vol. 19 (Jan-Jun 2010), pp. 205-213
Publication year: 2010
FOR Code(s): 0601 Biochemistry and Cell Biology; 0903 Biomedical Engineering
Keyword(s): Biofilms; Iomaterials-associated infections; Non-adhesive fouling; Polyethylene glycol brush coating; Polymer brush; Staphylococcus epidermidis; Tissue integration; U2OS osteoblast
Publisher: Swiss Society for Biomaterials
ISSN: 1473-2262
Publisher URL: http://www.ecmjournal.org/journal/papers/vol019/vol019a20.php
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