Home List of Titles Molecular investigation of a microbial mat associated with the Great Artesian Basin
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/232471
- Molecular investigation of a microbial mat associated with the Great Artesian Basin
- Byers, Helen K.; Stackebrandt, Erko; Hayward, Chris; Blackall, Linda L.
- Culture-independent modes of analysis were chosen to investigate a microbial mat associated with the thermal waters of the Great Artesian Basin (GAB). 16S rDNA was amplified from total genomic mat DNA, and used to construct a clone library. Use of plasmid-specific primers to amplify the inserts from 92 selected recombinants proved to be an effective approach, and demonstrated that there were four size categories of insert: 1500 bp (62 percent of clones examined), 1400 bp (25 percent of clones examined), 500 bp (5 percent of clones examined) and 240 bp (8 percent of clones examined). Restriction enzyme analysis was evaluated for its ability to group the 1500 bp and 1400 bp size inserts into operational taxonomic units. Clone inserts were presumptively identified by analysis of partial sequence data, and each operational taxonomic unit was found to be phylogenetically cohesive. Phylogenetic analyses of the sequence data indicated the presence of a broad range of bacteria related to the cyanobacteria, Thermus species, thiobacilli, planctomycetes, thermophilic hydrogen oxidisers, thermotogales, clostridia, actinomycetes, and β and δ subclasses of the proteobacteria. Use of the restriction enzyme analysis protocol also enabled the extent of insert repetition within the library to be monitored, and would thus have eliminated 70 percent of sequencing expenses. This is the first time a community analysis has been performed on this extreme environment.
- Publication type
- Journal article
- FEMS Microbiology Ecology, Vol. 25, no. 4 (Apr 1998), pp. 391-403
- Publication year
- FOR Code(s)
- 05 Environmental Sciences; 06 Biological Sciences; 11 Medical and Health Sciences
- Actinobacteria; Aquifer; Bacteria microorganisms; Clostridium; Cyanobacterium; Great Artesian Basin; Phylogeny; Planctomycetes; Prokaryota; Ribosome DNA; RNA 16S; Thermal spring; Thermophilic bacterium; Thermotogales; Thiobacillus
- Publisher URL
- Copyright © 1998 Federation of European Microbiological Societies. Published by Elsevier Science BV.
- Peer reviewed