Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/232523

Title

Phylogeny of the filamentous bacterium Eikelboom Type 1851, and design and application of a 16S rRNA targeted oligonucleotide probe for its fluorescence in situ identification in activated sludge

Author(s)

Beer, Michael;  Seviour, Elizabeth M.;  Kong, Yun;  Cunningham, Mitchell;  Blackall, Linda L.;  Seviour, Robert J.

Abstract

Micromanipulation was used to obtain an isolate (BEN 52) of Eikelboom Type 1851 from a bulking activated sludge plant. Its 16S rDNA sequence reveals its closest relative is 'Roseiflexus castenholzii', a member of the phylum 'Chloroflexi', class 'Chloroflexi', previously called the green non-sulfur bacteria. The 16S rRNA targeted oligonucleotide probe designed for fluorescence in situ hybridisation against this sequence successfully identified filamentous bacteria with the morphological features of Type 1851 in activated sludge samples from plants in several countries and different operational configurations.

Publication type

Journal article

Source

FEMS Microbiology Letters, Vol. 207, no. 2 (Feb 2002), pp. 179-183

Publication year

2002

FOR Code(s)

06 Biological Sciences;  07 Agricultural and Veterinary Sciences;  11 Medical and Health Sciences

Keyword(s)

Activated sludge;  Australia;  Bacterial RNA;  Chloroflexi;  DNA 16S;  Fluorescence in situ hybridization;  Green non-sulfur bacterium;  Kouleothrix aurantiaca;  Micromanipulation;  Morphology;  Nucleotide sequence;  Oligonucleotide;  Phylogeny;  Roseiflexus castenholzii

Publisher

Elsevier

ISSN

0378-1097

Publisher URL

http://dx.doi.org/10.1016/s0378-1097(01)00561-4

Copyright

Copright © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

Peer reviewed