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Home List of Titles Identification and comparison of aerobic and denitrifying polyphosphate-accumulating organisms
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/232485
- Identification and comparison of aerobic and denitrifying polyphosphate-accumulating organisms
- Zeng, Raymond J.; Saunders, Aaron M.; Yuan, Zhiguo; Blackall, Linda L.; Keller, Juerg
- Two laboratory-scale sequencing batch reactors (SBRs) were operated for enhanced biological phosphorus removal (EBPR) in alternating anaerobic-aerobic or alternating anaerobic-anoxic modes, respectively. Polyphosphate-accumulating organisms (PAOs) were enriched in the anaerobic-aerobic SBR and denitrifying PAOs (DPAOs) were enriched in the anaerobic-aerobic SBR. Fluorescence in situ hybridization (FISH) demonstrated that the well-known PAO, "Candidatus Accumulibacter phosphatis" was abundant in both SBRs, and post-FISH chemical staining with 4,6-diamidino-2phenylindol (DAPI) confirmed that they accumulated polyphosphate. When the anaerobic-anoxic SBR enriched for DPAOs was converted to anaerobic-aerobic operation, aerobic uptake of phosphorus by the resident microbial community occurred immediately. However, when the anaerobic-aerobic SBR enriched for PAOs was exposed to one cycle with anoxic rather than aerobic conditions, a 5-h lag period elapsed before phosphorus uptake proceeded. This anoxic phosphorus-uptake lag phase was not observed in the subsequent anaerobicaerobic cycle. These results demonstrate that the PAOs that dominated the anaerobic-aerobic SBR biomass were the same organisms as the DPAOs enriched under anaerobic-anoxic conditions.
- Publication type
- Journal article
- Biotechnology and Bioengineering, Vol. 83, no. 2 (Jul 2003), pp. 140-148
- Publication year
- Accumulibacter; Activated sludge; EBPR; Enhanced biological phosphorus removal; Denitrifying polyphosphate-accumulating organisms; DPAO; Fluorescence in situ hybridization; PAO; Polyphosphate-accumulating organisms; Sequencing batch reactors
- John Wiley & Sons
- Publisher URL
- Copyright © 2003 Wiley Periodicals Inc.
- Peer reviewed