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Collagen type I together with fibronectin provide a better support for endothelialization / Collagene de type I et fibronectine offrent un meilleur support pour favoriser la croissance de cellules endotheliales
List of Titles
Collagen type I together with fibronectin provide a better support for endothelialization / Collagene de type I et fibronectine offrent un meilleur support pour favoriser la croissance de cellules endotheliales
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/232856
- Title
- Collagen type I together with fibronectin provide a better support for endothelialization / Collagene de type I et fibronectine offrent un meilleur support pour favoriser la croissance de cellules endotheliales
- Author(s)
- Sgarioto, Melissa; Vigneron, Pascale; Patterson, John; Malherbe, Francois; Nagel, Marie-Danielle; Egles, Christophe
- Abstract
- Endothelialization of vascular implants is limited by the inability of cells to retain adhesion when exposed to flow. Extracellular matrix proteins, including fibronectin and collagen, enhance cell adherence on materials. This study investigated the behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) on extracellular matrix coated polystyrene. Collagen and fibronectin were coated as single and double layers to analyse differences in cell proliferation, morphology, and cell-protein interactions. Significantly higher endothelial cell proliferation and migration rates were observed on the collagen and collagen + fibronectin coating compared to the uncoated or fibronectin-coated sample. Immmunofluorescent microscopy showed evidence of extracellular matrix remodelling in the double, collagen + fibronectin coating. These results strongly suggest that a double coating of collagen + fibronectin provides a better support structure for endothelial cell growth and contributes to improve the ability of vascular implants to become and remain endothelialized.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Life and Social Sciences
- Source
- Comptes Rendus Biologies, Vol. 335, no. 8 (Aug 2012), pp. 520-528
- Publication year
- 2012
- FOR Code(s)
- 06 Biological Sciences
- Keyword(s)
- Collagen; Endothelialization; Extracellular matrix; Fibronectin; Vascular graft
- Publisher
- Elsevier
- Language
- English, French
- ISSN
- 1631-0691
- Publisher URL
- http://dx.doi.org/10.1016/j.crvi.2012.07.003
- Copyright
- Copyright © 2012 Academie des sciences. Published by Elsevier Masson SAS.
- Peer reviewed
