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Home List of Titles A colorimetric-based accurate method for the determination of enterovirus 71 titer
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/235673
- A colorimetric-based accurate method for the determination of enterovirus 71 titer
- Pourianfar, Hamid Reza; Javadi, Arman; Grollo, Lara
- The 50 % tissue culture infectious dose (TCID 50) is still one of the most commonly used techniques for estimating virus titers. However, the traditional TCID 50 assay is time consuming, susceptible to subjective errors and generates only quantal data. Here, we describe a colorimetric-based approach for the titration of Enterovirus 71 (EV71) using a modified method for making virus dilutions. In summary, the titration of EV71 using MTT or MTS staining with a modified virus dilution method decreased the time of the assay and eliminated the subjectivity of observational results, improving accuracy, reproducibility and reliability of virus titration, in comparison with the conventional TCID 50 approach (p<0.01). In addition, the results provided evidence that there was better correlation between a plaquing assay and our approach when compared to the traditional TCID 50 approach. This increased accuracy also improved the ability to predict the number of virus plaque forming units present in a solution. These improvements could be of use for any virological experimentation, where a quick accurate titration of a virus capable of causing cell destruction is required or a sensible estimation of the number of viral plaques based on TCID 50 of a virus is desired.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre
- Indian Journal of Virology, Vol. 23, no. 3 (Dec 2012), pp. 303-310
- Publication year
- Enterovirus 71; MTS; MTT; TCID50; Tissue culture infectious dose; Virus titration
- Publisher URL
- Copyright © Indian Virological Society 2012.
- Peer reviewed