Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach

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Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/236568

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Title: Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach
Author(s): Kirk, K.; Poh, C. L.; Fecondo, J.; Pourianfer, H.; Shaw, J.; Grollo, L.
Abstract: Currently, infections of hand, foot and mouth disease (HFMD) due to Human Enterovirus 71 (EV71) cannot be prevented or treated, as there are no suitable vaccines or antiviral drugs. This study aimed to identify potential vaccine candidates for EV71 using in silico analysis of its viral capsid proteins. A combined in silico approach utilizing computational hidden Markov model (HMM), propensity scale algorithm, and artificial learning, identified three 15-mer structurally conserved B-cell epitope candidates lying within the EV71 capsid proteins. Peptide vaccine candidates incorporating a target B-cell epitope and a promiscuous T-cell epitope from the related polio virus were synthesized using solid-phase Fmoc chemistry. Inbred BALB/C mice which were inoculated with two 10 μg doses of the synthetic peptide, generated anti-peptide antibodies. Purified IgG isolated from pooled sera of the inoculated mice neutralized EV71 infections in vitro. Furthermore, these neutralizing antibodies were cross-reactive against other members of the Picornaviridae family, demonstrating greater than 50% virus neutralization. This indicates that the current approach is promising for the development of synthetic peptide-based vaccine candidates against Picornaviridae. Development of effective vaccines is of paramount importance in managing the disease in the Asia Pacific regions where this virus is endemic and has significant social, economic and public health ramifications.
Publication type: Journal article
Research centre: Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre
Source: Vaccine, Vol. 30, no. 49 (Nov 2012), pp. 7105-7110
Publication year: 2012
FOR Code(s): 06 Biological Sciences; 07 Agricultural and Veterinary Sciences; 11 Medical and Health Sciences
Keyword(s): Antibodies; Enterovirus 71; Epitopes; In silico analysis; Neutralization; Synthetic peptides
Publisher: Elsevier
ISSN: 0264-410X
Publisher URL: http://dx.doi.org/10.1016/j.vaccine.2012.09.030
Peer reviewed