Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/5170

Title: Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation
Author(s): Xu, Ming G; Crimeen, Blessing; Ludford-Menting, Mandy J.; Gan, Xiaosong; Russell, Sarah M.; Gu, Min
Abstract: Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (FRET) in human cells under two-photon excitation was demonstrated in this study. A sample was prepared by expressing a donor and an acceptor in living cells and using an antibody to secure the proximity of contact between the donor and the acceptor. The quenching of fluorescence emission of a donor in the double-labelled cells indicates the presence of FRET that occurred in these living cells. Because of the quadratic relation of the excitation power, 3-D localisation of FRET becomes possible.
Publication type: Journal article
Research centre: Swinburne University of Technology. School of Biophysical Science
Source: Scanning, Vol. 23, no. 1 (2001), p. 9-13
Publication year: 2001
Keyword(s): 3-D microscopy; Fluorescence resonance energy transfer; Micro-spectroscopy; Two-photon microscopy
Format: 9-13
ISSN: 0161-0457
Publisher URL: http://dx.doi.org/10.1002/sca.4950230102
Peer reviewed