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Title

Mechanical strains induced in osteoblasts by use of point femtosecond laser targeting

Author(s)

Cranfield, Charles;  Bomzon, Ze'ev;  Day, Daniel;  Gu, Min;  Cartmell, Sarah

Abstract

A study demonstrating how ultrafast laser radiation stimulates osteoblasts is presented. The study employed a custom made optical system that allowed for simultaneous confocal cell imaging and targeted femtosecond pulse laser irradiation. When femtosecond laser light was focused onto a single cell, a rise in intracellular Ca 2+ levels was observed followed by contraction of the targeted cell. This contraction caused deformation of neighbouring cells leading to a heterogeneous strain field throughout the monolayer. Quantification of the strain fields in the monolayer using digital image correlation revealed local strains much higher than threshold values typically reported to stimulate extracellular bone matrix production in vitro. This use of point targeting with femtosecond pulse lasers could provide a new method for stimulating cell activity in orthopaedic tissue engineering.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

International Journal of Biomedical Imaging, Vol. 2006, no. (2006)

Publication year

2006

Publisher

Hindawi Publishing

ISSN

1687-4188

Publisher URL

http://dx.doi.org/10.1155/IJBI/2006/21304

Copyright

Copyright © 2006 Charles Cranfield et al. his is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Full text

Peer reviewed