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Title

High-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients

Author(s)

Day, Daniel;  Cranfield, Charles G.;  Gu, Min

Abstract

We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10-20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

International Journal of Biomedical Imaging, Vol. 2006, no. (2006), p. Article ID 93438

Publication year

2006

Publisher

Hindawi Publishing

ISSN

1687-4188

Publisher URL

http://dx.doi.org/10.1155/IJBI/2006/93438

Copyright

Copyright © 2006 Daniel Day et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Full text

Peer reviewed