Hierarchy of DNA immobilization and hybridization on poly-L-lysine : an atomic force microscopy study

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/5469

Title: Hierarchy of DNA immobilization and hybridization on poly-L-lysine : an atomic force microscopy study
Author(s): Sawant, Prashant D.; Nicolau, Dan V.
Abstract: Atomic force microscopy is used to analyze the immobilization of single-stranded DNA (ssDNA) on poly-L-lysine (PL)-coated glass and subsequent hybridization with complementary DNA (dsDNA). 2D fractal analysis (power spectrum density algorithm (PSDA)) and line analysis methods are used to characterize the vertical aggregation of ssDNA/dsDNA. The nanodefects on the PL layer could be responsible for the vertical aggregation of DNA. The line analysis suggests that approximately two layers of ssDNA are adsorbed, stacked vertically. The PSDA is useful in understanding the direction of aggregation in addition to understanding the growth mechanism. Fractal dimension values are between 2 and 3, suggesting that the growth mechanism may obey the diffusion-limited aggregation (DLA) model.
Publication type: Journal article
Research centre: Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. BioNanoEngineering Laboratory
Source: Smart Materials and Structures, Vol. 15, no. 1 (2006), p. S99-S103
Publication year: 2006
Publisher: IOP Publishing
Format: S99-S103
ISSN: 0964-1726
Publisher URL: http://dx.doi.org/10.1088/0964-1726/15/1/016
Peer reviewed