Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/5835

Title

Microcontact printing trapping air : a versatile tool for protein microarray fabrication

Author(s)

Filipponi, Luisa;  Nicolau, Dan V.

Abstract

The present work introduces a new method for the fabrication of protein micro-patterns, microcontact printing trapping air. The method is based on microcontact printing, a well-established soft-lithographic technique for printing bioactive protein patterns. Usually, the stamp used is made of poly(dimethylsiloxane) obtained by replicating a lithographically microfabricated silicon master. In microcontact printing, the dimensions of the features in the stamp are critical, since the high compressibility of poly(dimethylsiloxane) causes high aspect ratio features to collapse, leading to the printing of undesired areas. In most cases, this is an unwanted effect, which interferes with the printing quality. In this work we used a poly(dimethylsiloxane) stamp bearing an array of micro-posts which, when placed over a flat surface, collapses with consequent formation of an air gap around the entire array. This effect is linked to the distance between the posts that form the array and can be exploited for the fabrication of protein microarrays having a remarkably low background noise for fluorescence detection.

Publication type

Conference paper

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Industrial Research Institute Swinburne

Source

Proceedings of SPIE : Progress in Biomedical Optics and Imaging : BioMEMS and Nanotechnology II, Brisbane, Queensland, Australia, 12-14 December 2005, Vol. 6036, p. W1-W7

Publication year

2006

Keyword(s)

Microcontact printing;  Poly(dimethylsiloxane) microstructures;  Protein microarray;  Protein stamping;  Soft-lithography

Publisher

SPIE

Format

W1-W7

ISBN

0819460672

Publisher URL

http://dx.doi.org/10.1117/12.638463

Copyright

Copyright © 2006 SPIE - The International Society for Optical Engineering.

Peer reviewed