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Title

Characterization of gradient-index lens-fiber spacing toward applications in two-photon fluorescence endoscopy

Author(s)

Fu, Ling;  Gan, Xiaosong;  Gu, Min

Abstract

We report on the experimental investigation into the characterization of two-photon fluorescence microscopy based on the separation distance of a single-mode optical fiber coupler and a gradient-index (GRIN) rod lens. The collected two-photon fluorescence signal exhibits a maximum intensity at a defined separation distance (gap length) where the increasing effective excitation numerical aperture is balanced by the decreasing confocal emission collection. A maximum signal is found at gap lengths of approximately 2, 1.25, and 1.75 mm for GRIN lenses with pitches of 0.23, 0.25, and 0.29 wavelength at 830 nm. The maximum two-photon fluorescence signal collected corresponds to a threefold reduction of axial resolution (38.5 µm at 1.25 mm), compared with the maximum resolution (11.6 µm at 5.5 mm), as shown by the three-dimensional imaging of 10 µm beads. These results demonstrate an intrinsic trade-off between signal collection and axial resolution.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

Applied Optics, Vol. 44, no. 34 (2005), pp. 7270-7274

Publication year

2005

Publisher

Optical Society of America

ISSN

0003-6935

Publisher URL

http://www.opticsinfobase.org/abstract.cfm?id=86389

Copyright

Copyright © 2005 Optical Society of America. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?id=86389. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Full text

Peer reviewed