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Title

Reduced effects of spherical aberration on penetration depth under two-photon excitation

Author(s)

Ganic, Djenan;  Gan, Xiaosong;  Gu, Min

Abstract

We compare the effects of spherical aberration on the penetration depth of single-photon and two-photon excitation for instances in which the aberration is caused by the refractive-index mismatch when a beam is focused through an interface. It is shown both theoretically and experimentally that two-photon fluorescence imaging experiences less spherical aberration and can thus propagate to a deeper depth within a thick medium.

Publication type

Journal article

Research centre

Swinburne University of Technology. School of Biophysical Sciences and Electrical Engineering

Source

Applied Optics, Vol. 39, no. 22 (Aug 2000), pp. 3945-3947

Publication year

2000

Publisher

Optical Society of America

ISSN

0003-6935

Publisher URL

http://dx.doi.org/10.1364/AO.39.003945

Copyright

Copyright © 2000 Optical Society of America. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?URI=ao-39-22-3945. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Full text

Peer reviewed