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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/708
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- Comparison of penetration depth between two-photon excitation and single-photon excitation in imaging through turbid tissue media
- Gu, Min; Gan, Xiaosong; Kisteman, Aernout; Xu, Ming G.
- We show, both theoretically and experimentally, that for a turbid tissue medium where Mie scattering is dominant, multiple scattering not only reduces the illumination power in the forward direction but also exhibits an anisotropic distribution of scattered photons. Thus, a signal level under two-photon excitation drops much faster than that under single-photon excitation although image resolution is much higher in the former case. As a result, the penetration depth under two-photon excitation is limited by the strength of two-photon fluorescence and is not necessarily larger than that under single-photon excitation.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. School of Biophysical Sciences and Electrical Engineering. Centre for Micro-Photonics
- Applied Physics Letters, Vol. 77, no. 10 (Sept. 2000), pp. 1551-1553
- Publication year
- Biological tissues; Bioluminescence; Biomedical imaging; Bio-optics; Fluorescence; Light scattering; Optical images; Turbidity; Two-photon processes
- American Institute of Physics
- Publisher URL
- Copyright © 2000 American Institute of Physics. Reproduced in accordance with the copyright policy of the publisher.
- Full text
- Peer reviewed