Identification of immunodominant VP1 linear epitope of enterovirus71 (EV71) using synthetic peptides for detecting human anti-EV71 IgG antibodies in western blots

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Title: Identification of immunodominant VP1 linear epitope of enterovirus71 (EV71) using synthetic peptides for detecting human anti-EV71 IgG antibodies in western blots
Author(s): Foo, Damien G. W.; Ang, R. X.; Alonso, Sylvie; Chow, Vincent T. K.; Quak, Seng Hock; Poh, Chit Laa
Abstract: A major IgG-specific immunodominant VP1 linear epitope of enterovirus 71 (EV71) strain 41 (5865/SIN/00009), defined by the core sequence LEGTTNPNG, was identified by Pepscan analysis. Oligonucleotides corresponding to the amino-acid sequence of synthetic peptide SP32 were cloned and over-expressed in Escherichia coli as a recombinant glutathione-S-transferase (GST)-SP32 fusion protein. In ELISAs, this protein did not react with human anti-EV71 IgG antibodies, but there was significant immunoreactivity according to western blot analysis. The amino-acid sequence of SP32 was highly specific for detecting EV71 strains in western blot analysis, and showed no immunoreactivity with monoclonal antibodies raised against other enteroviruses, e.g., CA9 and Echo 6.
Publication type: Journal article
Research centre: Swinburne University of Technology. Faculty of Life and Social Sciences
Source: Clinical Microbiology and Infection, Vol. 14, no. 3 (Mar 2008), p. 286-288
Publication year: 2008
Keyword(s): Detection; Enterovirus 71; EV71; Immunodominant linear epitope; Pepscan analysis; Recombinant GST-SP32 fusion protein; Synthetic peptide
Publisher: Blackwell Publishing
ISSN: 1198-743X
Publisher URL: http://dx.doi.org/10.1111/j.1469-0691.2007.01904.x
Peer reviewed