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- Antibacterial compounds from Planchonia careya leaf extracts
- McRae, Jacqui M.; Yang, Qi; Crawford, Russell J.; Palombo, Enzo A.
- The leaves of Planchonia careya (F. Muell.) R. Knuth (Lecythidaceae) have been traditionally implemented in the treatment of wounds by the indigenous people of northern Australia, although the compounds responsible for the medicinal properties have not been identified. In this study, antibacterial compounds from the leaf extracts were isolated and characterized, and the biological activity of each compound was assessed. Compounds were isolated from the leaf extracts using HPLC-piloted activity-guided fractionation. The minimum inhibitory concentrations (MICs) were assessed with plate-hole diffusion assays, and the cytotoxicity was determined with MTT assays using monkey kidney epithelial (MA104) cells. Six known compounds were isolated from the leaf extracts and were identified as 1, (+)-gallocatechin; 2, gallocatechin-(4α → 8)-gallocatechin; 3, 9(S)-hydroxy-10E,12Z-octadecadienoic acid (α-dimorphecolic acid); 4, 2α,3β,24-trihydroxyolean-12-en-28-oic acid (hyptatic acid-A); 5, 3β-O-cis-p-coumaroyltormentic acid; and 6, 3β-O-trans-p-coumaroyltormentic acid. Compounds 5 and 6 were weakly selective for vancomycin-resistant Enterococcus (VRE) compared with eukaryotic cells, with an MIC of 59.4 μg/mL and a 50% inhibitory concentration (IC50) of 72.0 μg/mL for MA104 cells. The isolation of six antibacterial compounds from the leaves of Planchonia careya validates the use of this species as a topical wound-healing remedy.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre
- Journal of Ethnopharmacology, Vol. 116, no. 3 (Mar 2008), pp. 554-560
- Publication year
- FOR Code(s)
- 0607 Plant Biology; 1115 Pharmacology and Pharmaceutical Sciences
- Antibacterial activity; Lecythidaceae; Planchonia careya
- Publisher URL
- Copyright © 2008 Elsevier Ireland Ltd. All rights reserved. The accepted manuscript of the paper is reproduced here in accordance with the copyright policy of the publisher.
- Full text
- Peer reviewed