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Title

Fast handheld two-photon fluorescence microendoscope with a 475 mu m × 475 mu m field of view for in vivo imaging

Author(s)

Bao, H. C.;  Allen, John;  Pattie, Robert;  Vance, Rod;  Gu, Min

Abstract

A fast handheld two-photon fiber-optic fluorescence endoscope for three-dimensional (3D) in vivo cellular imaging is developed. The compact handheld probe of the two-photon endoscope can simply be placed into contact with the target tissue to reveal clear 3D surface and subsurface histological images without biopsy. The new system has, to the best of our knowledge, the largest field of view (FOV) of 475 μm × 475 μm and a 3D imaging volume larger than 475 μm × 475 μm × 250 μm. A real-time two-photon fluorescence image is displayed at 0.4 mm2/s. The lateral and axial resolutions of the two-photon fluorescence endoscope are better than 1 and 14.5 μm, respectively.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

Optics Letters, Vol. 33, no. 12 (Jun 2008), pp. 1333-1335

Publication year

2008

Keyword(s)

Fibre optics imaging;  Imaging systems;  Microscopy;  Nonlinear optics

Publisher

Optical Society of America

ISSN

0146-9592

Publisher URL

http://dx.doi.org/10.1364/OL.33.001333

Copyright

Copyright © 2008 Optical Society of America. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OL.33.001333. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Full text

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