This paper describes the use of a laser-based method to create microstructures within a bilayer system of gold and a non UV-sensitive polymer, poly(methyl methacrylate). Patterned surfaces were achieved through the use of a computer-controlled laser exposure system, comprising a research-grade inverted optical microscope, a pulsed nitrogen laser emitting at 337 nm and a programmable X-Y-Z stage. Those microstructures have proven to be useful for the adsorption of a variety of proteins. The technology has also been applied to an antigen-antibody specific recognition experiment, which proved the feasibility of this system for multianalyte protein assay. In the future, this system will be used as a platform for the optimisation of cell-cell communication within functionalised microstructures. In particular, the functionalisation of these micro-channels with neuropeptides is foreseen, with the aim of investigating effects that influence nerve regeneration.