Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/39722

Title

Polymer microstructures for multianalyte protein assay

Author(s)

Filipponi, Luisa;  Ivanova, Elena P.;  Wright, Jonathan P.;  Pham, Duy K.;  Nicolau, Dan V.

Abstract

This paper describes the use of a laser-based method to create microstructures within a bilayer system of gold and a non UV-sensitive polymer, poly(methyl methacrylate). Patterned surfaces were achieved through the use of a computer-controlled laser exposure system, comprising a research-grade inverted optical microscope, a pulsed nitrogen laser emitting at 337 nm and a programmable X-Y-Z stage. Those microstructures have proven to be useful for the adsorption of a variety of proteins. The technology has also been applied to an antigen-antibody specific recognition experiment, which proved the feasibility of this system for multianalyte protein assay. In the future, this system will be used as a platform for the optimisation of cell-cell communication within functionalised microstructures. In particular, the functionalisation of these micro-channels with neuropeptides is foreseen, with the aim of investigating effects that influence nerve regeneration.

Publication type

Conference paper

Research centre

Swinburne University of Technology. Industrial Research Institute Swinburne

Source

Profiles in Industrial Research: Knowledge and Innovation 2002, St Kilda, Victoria, Australia, 15-19 July 2002, pp. 308-312

Publication year

2002

Keyword(s)

Nerve regeneration;  Polymer microstructures;  Protein adsorption

Publisher

Industrial Research Institute Swinburne, Swinburne University of Technology

Publisher URL

http://www.swinburne.edu.au/feis/iris/publications.html