A rapid coupling protocol for the synthesis of peptide nucleic acids

Author(s)

Vearing, Christopher J.; Fecondo, John V.

Abstract

With the current interest in anti-sense and anti-gene technologies, an efficient, fast and less toxic synthesis protocol would be advantageous for the oligomerisation of Peptide Nucleic Acids (PNA). Most of the methods currently in use for the t-Boc synthesis of PNA's use TFA/m-cresol, pyridine, piperidine and capping reagents. In this work, a rapid synthesis protocol has been adapted from an earlier published peptide synthesis method allowing a reduction in cycle time from around 30 min down to 16 min. By utilising quantitative deprotection with 100% TFA, a coupling time of 10 min and a four-fold excess of monomer, this synthesis protocol has been used to synthesise a number of PNA's incorporating all four nucleotides of varying sequence, up to 17 residues in length.

Publication year

2002

Publication type

Journal article

Source

Letters in Peptide Science, Vol. 9, no. 4 (Jul 2002), pp. 211-219

ISSN

0929-5666

Publisher

Springer

Copyright

Copyright © 2003 Kluwer Academic Publishers.

Details